HPLC ASSAY FOR THE DETERMINATION OF ASCORBIC ACID IN HONEY SAMPLES

Abstract

Determination of ascorbic acid in honey samples was performed by high performance liquid chromatography (HPLC) with UV detection at 254 nm. The separation was performed using a C-18-ODS column with a mobile phase consisting of a mixture of 15% methanol and 85% water, adjusted to pH 2.5 with metaphosphoric acid. The mobile phase was pumped at a flow rate of 0.9 mL/min. The retention time for ascorbic acid was found to be 5.1 minutes. The method is simple, fast, sensitive, and reproducible. The recovery of ascorbic acid is over 90% by the standard addition method.