Wheat Flour Enzymatic Amylolysis Monitored by in Situ1H NMR Spectroscopy
- 23 January 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 52 (4), 823-831
- https://doi.org/10.1021/jf035188v
Abstract
Starch enzymatic degradation caused by endogenous hydrolases is studied by in situ NMR spectroscopy on a set of hard and soft wheat flours. The results obtained by two different techniques (HR-MAS and 1H NMR in solution) are analyzed in terms of a Michaelis−Menten kinetic phenomenological model taking into account the presence of endogenous enzymes and their eventual inactivation. The parameters resulting from the best fit of all experimental data to the kinetic model equations are submitted to a multivariate statistical analysis to assess the role of the oligosaccharides release in distinguishing between hard and soft wheats. Keywords: Wheat flour; hydrolyzing enzymes; 1H NMR; HR-MAS; statistical analysisThis publication has 8 references indexed in Scilit:
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