Stochastic transfer function for structured illumination microscopy.
- 19 June 2009
- journal article
- Published by Optica Publishing Group in Journal of the Optical Society of America A
- Vol. 26 (7), 1630-1637
- https://doi.org/10.1364/josaa.26.001630
Abstract
The stochastic transfer function extends the concept of the conventional transfer function by incorporating noise statistics, thus giving a measure of the signal-to-noise ratio at each spatial frequency. This provides a convenient and standardized metric to assess the trade-off in terms of spatial frequency bandwidth and signal-to-noise ratio for a diverse range of resolution enhancement techniques. We apply this concept to structured illumination microscopy and compare its noise performance as a function of frequency with the conventional wide-field fluorescent microscope. The result suggests how a hybrid algorithm further improves the photon efficiency of the structured illumination technique.Keywords
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