Stochastic transfer function: application to fluorescence microscopy
- 19 June 2009
- journal article
- Published by Optica Publishing Group in Journal of the Optical Society of America A
- Vol. 26 (7), 1622-1629
- https://doi.org/10.1364/josaa.26.001622
Abstract
We develop the concept of the stochastic transfer function (STF) and its application to high-resolution fluorescence microscopy. The STF is directly related to the conventional optical transfer functions but incorporates a probability density function at each spatial frequency. The mean of the STF yields the conventional transfer function; the variance of the STF gives a measure of the noise associated at each spatial frequency. The STF thus provides a more complete picture of the microscope performance in comparison with conventional transfer functions. Here we present the STF for a wide-field fluorescent microscope using both Monte Carlo simulation and probability theory.This publication has 10 references indexed in Scilit:
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