Metabolic Profiling of Strawberry Grape (Vitis × labruscana cv. ‘Isabella’) Components by Nuclear Magnetic Resonance (NMR) and Evaluation of Their Antioxidant and Antiproliferative Properties
- 9 June 2011
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 59 (14), 7679-7687
- https://doi.org/10.1021/jf200926t
Abstract
In the assessment of the antioxidant properties of edible plants, the widely consumed Vitis × labruscana cv. ‘Isabella’, known in Italy as “fragola” (strawberry) grape, was of interest. Phenol and flavonoid contents of the methanolic extracts of peel, pulp, seed, leaf, and stalk components of the plant were determined. The metabolic profile of the extracts was performed by 1D and 2D NMR. Quantitative analysis, obtained in the presence of 0.01% of internal standard trimethylsilyl propionate, evidenced the presence of catechins in both stalk and seed extracts, whereas caffeic acid and quercetin were the main metabolites of the leaf extract. Furthermore, the extracts were tested for their radical scavenging and reducing capacities by measuring their capacity to scavenge DPPH• and ABTS•+ and to reduce Fe(III) and Mo(VI) salts. The antioxidant efficacy of the extracts in cell-free systems and their antiproliferative activity toward HepG2 and A549 cells were also evaluated. Seed and stalk components are able to reduce by 39.6 and 40.6%, respectively, the amount of the metabolically active HepG2 cells after only 24 h of exposure.Keywords
This publication has 25 references indexed in Scilit:
- Spectroscopic Characterization and Antiproliferative Activity on HepG2 Human Hepatoblastoma Cells of Flavonoid C-Glycosides from Petrorhagia velutinaJournal of Natural Products, 2010
- Brazilian red wines made from the hybrid grape cultivar Isabel: Phenolic composition and antioxidant capacityAnalytica Chimica Acta, 2010
- Biological Activities of Polyphenols from GrapesInternational Journal of Molecular Sciences, 2010
- ‘Limoncella’ apple, an Italian apple cultivar: Phenolic and flavonoid contents and antioxidant activityFood Chemistry, 2007
- Free-Radical-Scavenging and Antioxidant Activities of Secondary Metabolites from Reddened Cv. Annurca Apple FruitsJournal of Agricultural and Food Chemistry, 2006
- Comparative Effects of Food-Derived Polyphenols on the Viability and Apoptosis of a Human Hepatoma Cell Line (HepG2)Journal of Agricultural and Food Chemistry, 2005
- Inhibition of Rat Mammary Tumorigenesis by Concord Grape Juice ConstituentsJournal of Agricultural and Food Chemistry, 2003
- Antioxidant Activity of Plant Extracts Containing Phenolic CompoundsJournal of Agricultural and Food Chemistry, 1999
- The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicalsFood Chemistry, 1999
- Comparative study of radical scavenger and antioxidant properties of phenolic compounds from Vitis vinifera cell cultures using in vitro testsLife Sciences, 1997