Evaluation of Catechin Synergistic and Antibacterial Efficacy on Biofilm Formation and acrA Gene Expression of Uropathogenic E. coli Clinical Isolates
Open Access
- 9 September 2022
- journal article
- research article
- Published by MDPI AG in Antibiotics
- Vol. 11 (9), 1223
- https://doi.org/10.3390/antibiotics11091223
Abstract
Uropathogenic Escherichia coli has a propensity to build biofilms to resist host defense and antimicrobials. Recurrent urinary tract infection (UTI) caused by multidrug-resistant, biofilm-forming E. coli is a significant public health problem. Consequently, searching for alternative medications has become essential. This study was undertaken to investigate the antibacterial, synergistic, and antibiofilm activities of catechin isolated from Canarium patentinervium Miq. against three E. coli ATCC reference strains (ATCC 25922, ATCC 8739, and ATCC 43895) and fifteen clinical isolates collected from UTI patients in Baghdad, Iraq. In addition, the expression of the biofilm-related gene, acrA, was evaluated with and without catechin treatment. Molecular docking was performed to evaluate the binding mode between catechin and the target protein using Autodock Vina 1.2.0 software. Catechin demonstrated significant bactericidal activity with a minimum inhibitory concentration (MIC) range of 1–2 mg/mL and a minimum bactericidal concentration (MBC) range of 2–4 mg/mL and strong synergy when combined with tetracycline at the MBC value. In addition, catechin substantially reduced E. coli biofilm by downregulating the acrA gene with a reduction percent ≥ 60%. In silico analysis revealed that catechin bound with high affinity (∆G = −8.2 kcal/mol) to AcrB protein (PDB-ID: 5ENT), one of the key AcrAB-TolC efflux pump proteins suggesting that catechin might inhibit the acrA gene indirectly by docking at the active site of AcrB protein.Keywords
This publication has 70 references indexed in Scilit:
- Anti-Inflammatory, Anticholinesterase, and Antioxidant Potential of Scopoletin Isolated fromCanarium patentinerviumMiq. (Burseraceae Kunth)Evidence-Based Complementary and Alternative Medicine, 2013
- Conserved small protein associates with the multidrug efflux pump AcrB and differentially affects antibiotic resistanceProceedings of the National Academy of Sciences of the United States of America, 2012
- Inhibition of Bacterial Biofilm Formation and Swarming Motility by a Small Synthetic Cationic PeptideAntimicrobial Agents and Chemotherapy, 2012
- A Real-Time PCR Antibiogram for Drug-Resistant SepsisPLOS ONE, 2011
- In Vitro Antimicrobial, Antioxidant Activities and Phytochemical Analysis of Canarium patentinervium Miq. from MalaysiaBiotechnology Research International, 2011
- Microtiter Dish Biofilm Formation AssayJournal of Visualized Experiments, 2011
- Optimized Nile Red Efflux Assay of AcrAB-TolC Multidrug Efflux System Shows Competition between SubstratesAntimicrobial Agents and Chemotherapy, 2010
- AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibilityJournal of Computational Chemistry, 2009
- Medical biofilmsBiotechnology & Bioengineering, 2008
- Pathogenic Escherichia coliNature Reviews Microbiology, 2004