Inhibition of Bacterial Biofilm Formation and Swarming Motility by a Small Synthetic Cationic Peptide
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Open Access
- 1 May 2012
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 56 (5), 2696-2704
- https://doi.org/10.1128/aac.00064-12
Abstract
Biofilms cause up to 80% of infections and are difficult to treat due to their substantial multidrug resistance compared to their planktonic counterparts. Based on the observation that human peptide LL-37 is able to block biofilm formation at concentrations below its MIC, we screened for small peptides with antibiofilm activity and identified novel synthetic cationic peptide 1037 of only 9 amino acids in length. Peptide 1037 had very weak antimicrobial activity, but at 1/30th the MIC the peptide was able to effectively prevent biofilm formation (>50% reduction in cell biomass) by the Gram-negative pathogens Pseudomonas aeruginosa and Burkholderia cenocepacia and Gram-positive Listeria monocytogenes . Using a flow cell system and a widefield fluorescence microscope, 1037 was shown to significantly reduce biofilm formation and lead to cell death in biofilms. Microarray and follow-up studies showed that, in P. aeruginosa , 1037 directly inhibited biofilms by reducing swimming and swarming motilities, stimulating twitching motility, and suppressing the expression of a variety of genes involved in biofilm formation (e.g., PA2204). Comparison of microarray data from cells treated with peptides LL-37 and 1037 enabled the identification of 11 common P. aeruginosa genes that have a role in biofilm formation and are proposed to represent functional targets of these peptides. Peptide 1037 shows promise as a potential therapeutic agent against chronic, recurrent biofilm infections caused by a variety of bacteria.Keywords
This publication has 62 references indexed in Scilit:
- Response rescaling in bacterial chemotaxisProceedings of the National Academy of Sciences of the United States of America, 2011
- Cell density and mobility protect swarming bacteria against antibioticsProceedings of the National Academy of Sciences of the United States of America, 2010
- Listeria monocytogenes — from saprophyte to intracellular pathogenNature Reviews Microbiology, 2009
- Pseudomonas aeruginosa biofilm formation in the cystic fibrosis airwayPulmonary Pharmacology & Therapeutics, 2008
- Dispersing biofilms with engineered enzymatic bacteriophageProceedings of the National Academy of Sciences of the United States of America, 2007
- Antibiotics as intermicrobial signaling agents instead of weaponsProceedings of the National Academy of Sciences of the United States of America, 2006
- Broad-spectrum biofilm inhibition by a secreted bacterial polysaccharideProceedings of the National Academy of Sciences of the United States of America, 2006
- The co-evolution of host cationic antimicrobial peptides and microbial resistanceNature Reviews Microbiology, 2006
- Aminoglycoside antibiotics induce bacterial biofilm formationNature, 2005
- Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?Nature Reviews Microbiology, 2005