BACTERIOCIN EXPOSURE AND FOOD INGREDIENTS INFLUENCE ON GROWTH AND VIRULENCE OF LISTERIA MONOCYTOGENES IN A MODEL MEAT GRAVY SYSTEM
- 1 October 2003
- journal article
- Published by Wiley in Journal of Food Safety
- Vol. 23 (3), 201-217
- https://doi.org/10.1111/j.1745-4565.2003.tb00363.x
Abstract
The antilisterial activity of bacteriocin-producing Lactobacillus sakei 1 bac+ alone and combined with food ingredients (sodium chloride, D-glucose, oregano, black pepper) was studied in a model meat gravy (1.8% proteose peptone, 1.2% meat extract, 0.6% yeast extract, 2.0% corn starch) kept under refrigeration for 10 days. Two strains of L. monocytogenes (serotypes 4b and 1/2a) were employed in coinoculation experiments and Lactobacillus sakei ATCC 15521 was used as a negative control for bacteriocin production. The LAB bac+ strain was more effective in inhibiting both L. monocytogenes serotypes than the LAB bac− strain. The serotype 4b was more sensitive to bacteriocin than serotype 1/2a. The effect of the ingredients on inhibition of L. monocytogenes was serotype dependent. Bacteriocin exposure did not affect sensitivity to ampicilin and rifampicin. However, L. monocytogenes partially lost their hemolytic activity after exposure to bacteriocin-producing Lb. sakei 1 and food ingredients.Keywords
This publication has 23 references indexed in Scilit:
- Identification of meat isolated bacteriocin-producing lactic acid bacteria using biotyping and ribotypingArquivo Brasileiro de Medicina Veterinária e Zootecnia, 2002
- Nisin: A Novel Substrate for Glutathione S‐Transferase Isolated from Fresh BeefJournal of Food Science, 2002
- Anti-Listeria monocytogenes activity of essential oils components of conifersFood Control, 2002
- Can food-related environmental factors induce different behaviour in two key serovars, 4b and 1/2a, of Listeria monocytogenes?International Journal of Food Microbiology, 2001
- In vitro activities of 22 antimicrobial agents against Listeria monocytogenes strains isolated in Barcelona, SpainDiagnostic Microbiology and Infectious Disease, 2000
- Behaviour of Listeria monocytogenes and autochthonous flora on meat stored under aerobic, vacuum and modified atmosphere packaging conditions with or without the presence of oregano essential oil at 5 oCJournal of Applied Microbiology, 2000
- An Outbreak of Febrile Gastroenteritis Associated with Corn Contaminated byListeria monocytogenesNew England Journal of Medicine, 2000
- Bacteriocinogenic lactic acid bacteria for the biopreservation of meat and meat productsMeat Science, 1998
- Insufficient antilisterial capacity of low inoculum Lactobacillus cultures on long-term stored meats at 4 °CInternational Journal of Food Microbiology, 1997
- Evaluation of phenotypic markers associated with pathogenicity in the genus ListeriaRevista do Instituto de Medicina Tropical de São Paulo, 1994