Controllable Self-Assembly of Nanoparticles for Specific Delivery of Multiple Therapeutic Molecules to Cancer Cells Using RNA Nanotechnology
- 1 September 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 5 (9), 1797-1808
- https://doi.org/10.1021/nl051264s
Abstract
By utilizing RNA nanotechnology, we engineered both therapeutic siRNA and a receptor-binding RNA aptamer into individual pRNAs of phi29's motor. The RNA building block harboring siRNA or other therapeutic molecules was fabricated subsequently into a trimer through the interaction of engineered right and left interlocking RNA loops. The incubation of the protein-free nanoscale particles containing the receptor-binding aptamer or other ligands resulted in the binding and co-entry of the trivalent therapeutic particles into cells, subsequently modulating the apoptosis of cancer cells and leukemia model lymphocytes in cell culture and animal trials. The use of such antigenicity-free 20−40 nm particles holds promise for the repeated long-term treatment of chronic diseases.This publication has 58 references indexed in Scilit:
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