DNA Polymerase-Mediated DNA Synthesis on a TNA Template
Open Access
- 28 December 2002
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 125 (4), 856-857
- https://doi.org/10.1021/ja028589k
Abstract
TNA, or threose nucleic acid, is capable of Watson−Crick base pairing with DNA, RNA, and TNA; coupled with its chemical simplicity, this suggests that TNA is a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for activity on a TNA template. We report that despite having a repeating unit that is one atom shorter than that of DNA, several polymerases showed surprisingly good ability to copy limited stretches of TNA.Keywords
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