Optimization of the Extraction of Anthocyanins from the Fruit Skin of Rhodomyrtus tomentosa (Ait.) Hassk and Identification of Anthocyanins in the Extract Using High-Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry (HPLC-ESI-MS)
Open Access
- 22 May 2012
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 13 (5), 6292-6302
- https://doi.org/10.3390/ijms13056292
Abstract
Anthocyanins are naturally occurring polyphenols that impart bright color to fruits, vegetables and plants. In this study, the extraction of anthocyanins from freeze-dried fruit skin of downy rose-myrtle (Rhodomyrtus tomentosa (Ait.) Hassk var. Gangren) was optimized using response surface methodology (RSM). Using 60% ethanol containing 0.1% (v/v) hydrochloric acid as extraction solvent, the optimal conditions for maximum yields of anthocyanin (4.358 ± 0.045 mg/g) were 15.7:1 (v/w) liquid to solid ratio, 64.38 °C with a 116.88 min extraction time. The results showed good fits with the proposed model for the anthocyanin extraction (R2 = 0.9944). Furthermore, the results of high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) analysis of the anthocyanins extracted from the fruit skin of downy rose-myrtle revealed the presence of five anthocyanin components, which were tentatively identified as delphinidin-3-glucoside, cyanidin-3-glucoside, peonidin-3-glucoside, petunidin-3-glucoside and malvidin-3-glucoside.This publication has 19 references indexed in Scilit:
- Optimization of Ultrasound-Assisted Extraction of Anthocyanins from Mulberry, Using Response Surface MethodologyInternational Journal of Molecular Sciences, 2011
- Optimization of microwave-assisted extraction of anthocyanins from purple corn (Zea mays L.) cob and identification with HPLC–MSInnovative Food Science & Emerging Technologies, 2010
- Anthocyanins: Natural Colorants with Health-Promoting PropertiesAnnual Review of Food Science and Technology, 2010
- A 90-day oral toxicity study of purple corn color, a natural food colorant, in F344 ratsFood and Chemical Toxicology, 2008
- Application of response surface methodology to cell immobilization for the production of palatinoseBioresource Technology, 2007
- Anthocyanin dyes extracted from grape pomace for the purpose of textile dyeingJournal of the Science of Food and Agriculture, 2007
- Anthocyanins in Wild Blueberries of Quebec: Extraction and IdentificationJournal of Agricultural and Food Chemistry, 2007
- Contents of Anthocyanins and Ellagitannins in Selected Foods Consumed in FinlandJournal of Agricultural and Food Chemistry, 2007
- Concentrations of Anthocyanins in Common Foods in the United States and Estimation of Normal ConsumptionJournal of Agricultural and Food Chemistry, 2006
- Application of statistical experimental design for optimization of alkaline protease production from Bacillus sp. RGR-14Process Biochemistry, 2004