Anthocyanins in Wild Blueberries of Quebec: Extraction and Identification
- 19 June 2007
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 55 (14), 5626-5635
- https://doi.org/10.1021/jf0703304
Abstract
Anthocyanins were extracted from a mixture of berries of Vaccinium angustifolium and Vaccinium myrtilloïdes at 7.7 °C, 26 °C, and 79 °C using ethanol alone or ethanol acidified with hydrochloric, citric, tartaric, lactic, or phosphoric acids at a solvent to solid ratio of 10. The effect of these parameters on extracted anthocyanins stability was investigated. The pH-differential and HPLC-DAD methods were used to determine anthocyanin contents. Extracted anthocyanins were purified on a C-18 solid-phase extraction cartridge and characterized by HPLC/electrospray ionization/mass spectrometry (HPLC-ESI-MS/MS). Anthocyanins were identified according to their HPLC retention times, elution order, and MS fragmentation pattern and by comparison with standards and published data. Anthocyanin extractions gave different yields depending on the type of added acid and the extraction temperature. High yields of monomeric and total anthocyanins (26.3 and 28.9 mg/g of dry matter) were obtained at 79 °C using phosphoric acid. Extraction using tartaric acid at 79 °C provided the lowest degradation index (1.05). Anthocyanins were stable and browning by polyphenol oxidase was inhibited under these conditions. Of the six common anthocyanindins, five were identified in the extracts, namely, delpinidin, cyanidin, peonidin, petunidin, and malvidin; pelargonidin was not found. In addition to well-known major anthocyanins, new anthocyanins were identified for the first time in extracts of wild blueberries from Quebec. Keywords: Vaccinium angustifolium; Vaccinium myrtilloïdes; anthocyanins; extraction; solid-phase extraction; pH differential; HPLC-DAD; degradation index; HPLC-ESI-MS/MSThis publication has 8 references indexed in Scilit:
- Oxygen Radical Absorbance Capacity (ORAC) and Phenolic and Anthocyanin Concentrations in Fruit and Leaf Tissues of Highbush BlueberryJournal of Agricultural and Food Chemistry, 2001
- Antioxidant Capacity, Vitamin C, Phenolics, and Anthocyanins after Fresh Storage of Small FruitsJournal of Agricultural and Food Chemistry, 1999
- Antioxidant Capacity As Influenced by Total Phenolic and Anthocyanin Content, Maturity, and Variety ofVacciniumSpeciesJournal of Agricultural and Food Chemistry, 1998
- The relative contributions of ascorbic acid and phenolic antioxidants to the total antioxidant activity of orange and apple fruit juices and blackcurrant drinkFood Chemistry, 1997
- Mechanism of Browning in Fresh Highbush Blueberry Fruit (Vaccinium corymbosum L). Role of Blueberry Polyphenol Oxidase, Chlorogenic Acid and AnthocyaninsJournal of the Science of Food and Agriculture, 1997
- Computer-aided evaluation of the relative stability of Vaccinium myrtillus anthocyaninsAnalytica Chimica Acta, 1992
- Food colorants: AnthocyaninsCritical Reviews in Food Science and Nutrition, 1989
- Fruit Color Loss, Effects of Carbohydrates and Other Factors on Strawberry ProductsJournal of Agricultural and Food Chemistry, 1953