Distribution of UV light-induced damage in a defined sequence of human DNA: detection of alkaline-sensitive lesions at pyrimidine nucleoside-cytidine sequences.
- 1 June 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (6), 3388-3392
- https://doi.org/10.1073/pnas.78.6.3388
Abstract
The distribution of UV light-induced damage to the highly reiterated .alpha. sequence of human DNA was investigated. The distribution of UV light-induced cyclobutane dimers within a defined sequence is similar whether the DNA is exposed to UV light as part of the chromosome of intact cells [cervical carcinoma HeLa cells, CEM lymphoblastic leukemia and Tk6 .beta. lymphocytes]. The cellular environment shields the nuclear DNA, resulting in about 50% decrease in apparent dose. A new type of UV photodamage was detected. Treatment of UV light-irradiated DNA with hot alkali results in strand breaks at positions of cytidine located 3'' to pyrimidine nucleosides. The chemical nature and biological significance of the pyrimidine nucleoside-cytidine lesion is discussed.This publication has 22 references indexed in Scilit:
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