The effect of the macrolide antibiotic tylosin on microbial diversity in the canine small intestine as demonstrated by massive parallel 16S rRNA gene sequencing
Top Cited Papers
Open Access
- 1 January 2009
- journal article
- Published by Springer Science and Business Media LLC in BMC Microbiology
- Vol. 9 (1), 210
- https://doi.org/10.1186/1471-2180-9-210
Abstract
Recent studies have shown that the fecal microbiota is generally resilient to short-term antibiotic administration, but some bacterial taxa may remain depressed for several months. Limited information is available about the effect of antimicrobials on small intestinal microbiota, an important contributor to gastrointestinal health. The antibiotic tylosin is often successfully used for the treatment of chronic diarrhea in dogs, but its exact mode of action and its effect on the intestinal microbiota remain unknown. The aim of this study was to evaluate the effect of tylosin on canine jejunal microbiota. Tylosin was administered at 20 to 22 mg/kg q 24 hr for 14 days to five healthy dogs, each with a pre-existing jejunal fistula. Jejunal brush samples were collected through the fistula on days 0, 14, and 28 (14 days after withdrawal of tylosin). Bacterial diversity was characterized using massive parallel 16S rRNA gene pyrosequencing.Keywords
This publication has 39 references indexed in Scilit:
- Differential Effects of Bifidobacterium pseudolongum Strain Patronus and Metronidazole in the Rat GutApplied and Environmental Microbiology, 2009
- The Pervasive Effects of an Antibiotic on the Human Gut Microbiota, as Revealed by Deep 16S rRNA SequencingPLoS Biology, 2008
- Bacterial Tag–Encoded FLX Amplicon Pyrosequencing (bTEFAP) for Microbiome Studies: Bacterial Diversity in the Ileum of Newly WeanedSalmonella-Infected PigsFoodborne Pathogens & Disease, 2008
- A Comparative Study on the Effects of Tylosin on Select Bacteria During Continuous Flow Culture of Mixed Populations of Gut Microflora Derived from a Feral and a Domestic PigFoodborne Pathogens & Disease, 2008
- The Macaque Gut Microbiome in Health, Lentiviral Infection, and Chronic EnterocolitisPLoS Pathogens, 2008
- Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP)BMC Microbiology, 2008
- Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseasesProceedings of the National Academy of Sciences of the United States of America, 2007
- Pyrosequencing enumerates and contrasts soil microbial diversityThe ISME Journal, 2007
- Adherent and InvasiveEscherichia coliIs Associated with Granulomatous Colitis in Boxer DogsInfection and Immunity, 2006
- UniFrac: a New Phylogenetic Method for Comparing Microbial CommunitiesApplied and Environmental Microbiology, 2005