Evaluation of the bacterial diversity in the feces of cattle using 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP)
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Open Access
- 1 January 2008
- journal article
- Published by Springer Science and Business Media LLC in BMC Microbiology
- Vol. 8 (1), 125
- https://doi.org/10.1186/1471-2180-8-125
Abstract
The microbiota of an animal's intestinal tract plays important roles in the animal's overall health, productivity and well-being. There is still a scarcity of information on the microbial diversity in the gut of livestock species such as cattle. The primary reason for this lack of data relates to the expense of methods needed to generate such data. Here we have utilized a bacterial tag-encoded FLX 16s rDNA amplicon pyrosequencing (bTEFAP) approach that is able to perform diversity analyses of gastrointestinal populations. bTEFAP is relatively inexpensive in terms of both time and labor due to the implementation of a novel tag priming method and an efficient bioinformatics pipeline. We have evaluated the microbiome from the feces of 20 commercial, lactating dairy cows. Ubiquitous bacteria detected from the cattle feces included Clostridium, Bacteroides, Porpyhyromonas, Ruminococcus, Alistipes, Lachnospiraceae, Prevotella, Lachnospira, Enterococcus, Oscillospira, Cytophage, Anaerotruncus, and Acidaminococcus spp. Foodborne pathogenic bacteria were detected in several of the cattle, a total of 4 cows were found to be positive for Salmonella spp (tentative enterica) and 6 cows were positive for Campylobacter spp. (tentative lanienae). Using bTEFAP we have examined the microbiota in the feces of cattle. As these methods continue to mature we will better understand the ecology of the major populations of bacteria the lower intestinal tract. This in turn will allow for a better understanding of ways in which the intestinal microbiome contributes to animal health, productivity and wellbeing.Keywords
This publication has 67 references indexed in Scilit:
- The characterization of lactic acid producing bacteria from the rumen of dairy cattle grazing on improved pasture supplemented with wheat and barley grainJournal of Applied Microbiology, 2008
- Comparison of In Vitro Fermentation and Molecular Microbial Profiles of High-Fiber Feed Substrates Incubated with Chicken Cecal InoculaPoultry Science, 2007
- Outbreak of multidrug-resistant Salmonella newport--United States, January-April 2002.2002
- Extensive Set of 16S rRNA-Based Probes for Detection of Bacteria in Human FecesApplied and Environmental Microbiology, 2002
- In vitro expression of tumor necrosis factor-alpha, interleukin 1beta, and interleukin 8 mRNA by bovine macrophages following exposure to Porphyromonas levii.2002
- Conventional and Molecular Methods for Understanding Probiotic Bacteria Functionality in Gastrointestinal TractsCritical Reviews in Microbiology, 1999
- Autism and clostridium tetaniMedical Hypotheses, 1998
- The rumen microbial ecosystem—some recent developmentsTrends in Microbiology, 1997
- Isolation and Characterization of Rice-Straw Degrading Clostridia from Cattle Rumen.The Journal of Veterinary Medical Science, 1992
- Influence of Feeding Fermented Colostrum and Lactobacillus acidophilus on Fecal Flora of Dairy CalvesJournal of Dairy Science, 1980