Epigenetic rather than genetic factors may explain phenotypic divergence between coastal populations of diploid and tetraploid Limonium spp. (Plumbaginaceae) in Portugal
Open Access
- 6 December 2013
- journal article
- Published by Springer Science and Business Media LLC in BMC Plant Biology
- Vol. 13 (1), 205
- https://doi.org/10.1186/1471-2229-13-205
Abstract
Background: The genus Limonium Miller comprises annual and perennial halophytes that can produce sexual and/or asexual seeds (apomixis). Genetic and epigenetic (DNA methylation) variation patterns were investigated in populations of three phenotypically similar putative sexual diploid species (L. nydeggeri, L. ovalifolium, L. lanceolatum), one sexual tetraploid species (L. vulgare) and two apomict tetraploid species thought to be related (L. dodartii, L. multiflorum). The extent of morphological differentiation between these species was assessed using ten diagnostic morphometric characters. Results: A discriminant analysis using the morphometric variables reliably assigns individuals into their respective species groups. We found that only modest genetic and epigenetic differentiation was revealed between species by Methylation Sensitive Amplification Polymorphism (MSAP). However, whilst there was little separation possible between ploidy levels on the basis of genetic profiles, there was clear and pronounced interploidy discrimination on the basis of epigenetic profiles. Here we investigate the relative contribution of genetic and epigenetic factors in explaining the complex phenotypic variability seen in problematic taxonomic groups such as Limonium that operate both apomixis and sexual modes of reproduction. Conclusions: Our results suggest that epigenetic variation might be one of the drivers of the phenotypic divergence between diploid and tetraploid taxa and discuss that intergenome silencing offers a plausible mechanistic explanation for the observed phenotypic divergence between these microspecies. These results also suggest that epigenetic profiling offer an additional tool to infer ploidy level in stored specimens and that stable epigenetic change may play an important role in apomict evolution and species recognition.Keywords
This publication has 58 references indexed in Scilit:
- Male fertility versus sterility, cytotype, and DNA quantitative variation in seed production in diploid and tetraploid sea lavenders (Limonium sp., Plumbaginaceae) reveal diversity in reproduction modesSexual Plant Reproduction, 2012
- Bringing Together Evolution on Serpentine and Polyploidy: Spatiotemporal History of the Diploid-Tetraploid Complex of Knautia arvensis (Dipsacaceae)PLOS ONE, 2012
- Amplified Fragment Length Polymorphism: An Invaluable Fingerprinting Technique for Genomic, Transcriptomic, and Epigenetic StudiesPublished by Springer Science and Business Media LLC ,2012
- The more the better? The role of polyploidy in facilitating plant invasionsAnnals of Botany, 2011
- Methylation changes associated with early maturation stages in the Atlantic salmonBMC Genetics, 2011
- Epigenetic Variation in Mangrove Plants Occurring in Contrasting Natural EnvironmentPLOS ONE, 2010
- Chromatin structure and the inheritance of epigenetic informationNature Reviews Genetics, 2010
- Establishing, maintaining and modifying DNA methylation patterns in plants and animalsNature Reviews Genetics, 2010
- Evolutionary Genetics of Genome Merger and Doubling in PlantsAnnual Review of Genetics, 2008
- genalex 6: genetic analysis in Excel. Population genetic software for teaching and researchMolecular Ecology Notes, 2005