Amplified Fragment Length Polymorphism: An Invaluable Fingerprinting Technique for Genomic, Transcriptomic, and Epigenetic Studies
- 7 February 2012
- book chapter
- research article
- Published by Springer Science and Business Media LLC
- Vol. 862, 75-87
- https://doi.org/10.1007/978-1-61779-609-8_7
Abstract
Amplified fragment length polymorphism (AFLP) is a PCR-based technique that uses selective amplification of a subset of digested DNA fragments to generate and compare unique fingerprints for genomes of interest. The power of this method relies mainly in that it does not require prior information regarding the targeted genome, as well as in its high reproducibility and sensitivity for detecting polymorphism at the level of DNA sequence. Widely used for plant and microbial studies, AFLP is employed for a variety of applications, such as to assess genetic diversity within species or among closely related species, to infer population-level phylogenies and biogeographic patterns, to generate genetic maps, and to determine relatedness among cultivars. Variations of standard AFLP methodology have been also developed for targeting additional levels of diversity, such as transcriptomic variation and DNA methylation polymorphism.Keywords
This publication has 12 references indexed in Scilit:
- Stable Epigenetic Effects Impact Adaptation in Allopolyploid Orchids (Dactylorhiza: Orchidaceae)Molecular Biology and Evolution, 2010
- Genetic and epigenetic alterations after hybridization and genome doubling2007
- The generalist flower deconstructedTaxon, 2007
- Genetic and epigenetic consequences of recent hybridization and polyploidy in Spartina (Poaceae)Molecular Ecology, 2005
- How to track and assess genotyping errors in population genetics studiesMolecular Ecology, 2004
- Using SD-AFLP and MSAP to assess CCGG methylation in the banana genomePlant Molecular Biology Reporter, 2003
- Hybridization, polyploidy and speciation inSpartina(Poaceae)New Phytologist, 2003
- Quantitative cDNA-AFLP analysis for genome-wide expression studiesMolecular Genetics and Genomics, 2003
- Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber developmentThe Plant Journal, 1996
- AFLP: a new technique for DNA fingerprintingNucleic Acids Research, 1995