Twin-Airy Point-Spread Function for Extended-Volume Particle Localization
Open Access
- 15 May 2020
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review Letters
- Vol. 124 (19), 198104
- https://doi.org/10.1103/physrevlett.124.198104
Abstract
The localization of point sources in optical microscopy enables nm-precision imaging of single-molecules and biological dynamics. We report a new method of localization microscopy using twin Airy beams that yields precise 3D localization with the key advantages of extended depth range, higher optical throughput, and potential for imaging higher emitter densities than are possible using other techniques. A precision of better than 30 nm was achieved over a depth range in excess of using a , 1.4 NA objective. An illustrative application to extended-depth-range blood-flow imaging in a live zebrafish is also demonstrated.
Keywords
Funding Information
- Engineering and Physical Sciences Research Council (EP/K503058/1, EP/M506539/1)
- China Scholarship Council (201503170229)
- Scottish Universities Physics Alliance
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