Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions
Open Access
- 18 May 2015
- journal article
- letter
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 15 (6), 4194-4199
- https://doi.org/10.1021/acs.nanolett.5b01396
Abstract
We employ a novel framework for information-optimal microscopy to design a family of point spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of nanoscale emitters in three dimensions over customizable axial (z) ranges of up to 20 μm with a high numerical aperture objective lens. To illustrate, we perform flow profiling in a microfluidic channel and show scan-free tracking of single quantum-dot-labeled phospholipid molecules on the surface of living, thick mammalian cells.Keywords
Funding Information
- National Eye Institute (PN2EY016525)
- National Institute of General Medical Sciences (R01GM085437)
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