High-speed extended-volume blood flow measurement using engineered point-spread function
Open Access
- 26 November 2018
- journal article
- research article
- Published by Optica Publishing Group in Biomedical Optics Express
- Vol. 9 (12), 6444-6454
- https://doi.org/10.1364/boe.9.006444
Abstract
Experimental characterization of blood flow in living organisms is crucial for understanding the development and function of cardiovascular systems, but there has been no technique reported for snapshot imaging of thick samples in large volumes with high precision. We have combined computational microscopy and the diffraction-free, self-bending property of Airy-beams to track fluorescent beads with sub-micron precision through an extended axial range (up to 600 \textmu m) within the flowing blood of 3 days post-fertilization (dpf) zebrafish embryos. The spatial trajectories of the tracer beads within flowing blood were recorded during transit through both cardinal and intersegmental vessels, and the trajectories were found to be consistent with the segmentation of the vasculature recorded using selective-plane illumination microscopy (SPIM). This method provides sufficiently precise spatial and temporal measurement of 3D blood flow that has the potential for directly probing key biomechanical quantities such as wall shear stress, as well as exploring the fluidic repercussions of cardiovascular diseases. Although we demonstrate the technique for blood flow, the ten-fold better enhancement in the depth range offers improvements in a wide range of applications of high-speed precision measurement of fluid flow, from microfluidics through measurement of cell dynamics to macroscopic aerosol characterizations.Keywords
Funding Information
- British Heart Foundation (NH/14/2/31074)
- China Scholarship Council (201503170229)
- Engineering and Physical Sciences Research Council (EP/M506539/1, EP/K503058/1)
This publication has 41 references indexed in Scilit:
- In Vivo Wall Shear Measurements within the Developing Zebrafish HeartPLOS ONE, 2013
- High Resolution Imaging of Vascular Function in ZebrafishPLOS ONE, 2012
- Measurement of mechanical tractions exerted by cells in three-dimensional matricesNature Methods, 2010
- Cavitation effect of holmium laser pulse applied to ablation of hard tissue underwaterJournal of Biomedical Optics, 2010
- Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread functionProceedings of the National Academy of Sciences, 2009
- Requirement of vasculogenesis and blood circulation in late stages of liver growth in zebrafishBMC Developmental Biology, 2008
- The environmental toxicant 2,3,7,8-tetrachlorodibenzo-p-dioxin disrupts morphogenesis of the rat pre-implantation embryoBMC Developmental Biology, 2008
- Three-dimensional real-time imaging of cardiac cell motions in living embryosJournal of Biomedical Optics, 2008
- Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)Nature Methods, 2006
- Methods of Digital Video Microscopy for Colloidal StudiesJournal of Colloid and Interface Science, 1996