Verbascosides from Olive Mill Waste Water: Assessment of Their Bioaccessibility and Intestinal Uptake Using an In Vitro Digestion/Caco‐2 Model System
- 1 February 2011
- journal article
- Published by Wiley in Journal of Food Science
- Vol. 76 (2), H48-H54
- https://doi.org/10.1111/j.1750-3841.2010.01996.x
Abstract
Olive mill wastewater (OMWW) is an agricultural waste material produced in high quantities in the Mediterranean basin. OMWW may be an inexpensive source of health promoting phytochemicals with potential economic value including many low molecular weight compounds such as verbascosides. While promising as antioxidants in vitro, little information is available on the potential absorption of verbascosides by humans. The main objective of the present study was to characterize the verbascoside content and potential for their bioavailability from a partially purified phenolic fraction (IP) of OMWW. The IP was obtained after ultrafiltration step at 5000 Dalton and gel filtration low-pressure chromatography (LH20) of OMWW. RP-HPLC analysis identified several soluble phenolics compounds including verbascoside and isoverbascoside as major components of OMWW fractions. The potential for bioavailability of these polyphenols was estimated by using both in vitro digestion and Caco-2 human intestinal cell models. In vitro digestive recoveries (bioaccessibility) were found to be 35.5%± 0.55% for verbascoside and 9.2% ± 0.94% for isoverbascoside highlighting potential sensitivity of these phenolics to gastric and small intestinal digestive conditions. Accumulation of verbascosides by highly differentiated Caco-2 monolayers was linear between 10 and 100 μM of verbascoside and isoverbascoside from IP extract. Uptake of verbascoside and isoverbascoside was rapid with peak accumulation occurring after 30 min with total accumulation efficiency of 0.1% and 0.2% providing intracellular levels of 130 and 80 pmol/mg cell protein for verbascoside and isoverbascoside, respectively. Combined, these data suggest that verbascosides present in OMWW are bioaccessible and provides a rationale for subsequent in vivo studies on the bioavailability and bioactivity of OMWW components.Keywords
This publication has 43 references indexed in Scilit:
- Formulation with ascorbic acid and sucrose modulates catechin bioavailability from green teaFood Research International, 2010
- Olive Phenolics Increase Glutathione Levels in Healthy VolunteersJournal of Agricultural and Food Chemistry, 2009
- Chemistry and Bioactivity of Olive Biophenols in Some Antioxidant and Antiproliferative in Vitro BioassaysChemical Research in Toxicology, 2008
- Common tea formulations modulate in vitro digestive recovery of green tea catechinsMolecular Nutrition & Food Research, 2007
- Anthocyanins from red wine – Their stability under simulated gastrointestinal digestionPhytochemistry, 2005
- Bioavailability and antioxidant effects of olive oil phenols in humans: a reviewEuropean Journal of Clinical Nutrition, 2004
- Studies on the Antioxidant Activity of Lippia citriodora Infusion: Scavenging Effect on Superoxide Radical, Hydroxyl Radical and Hypochlorous AcidBiological & Pharmaceutical Bulletin, 2002
- Simulated intestinal digestion of green and black teasFood Chemistry, 2001
- Antioxidative Activities of Phenylethanoid Glycosides from Ligustrum purpurascensJournal of Agricultural and Food Chemistry, 2001
- Free Radical-Scavenging Properties of Olive Oil PolyphenolsBiochemical and Biophysical Research Communications, 1998