Chemistry and Bioactivity of Olive Biophenols in Some Antioxidant and Antiproliferative in Vitro Bioassays
- 19 December 2008
- journal article
- Published by American Chemical Society (ACS) in Chemical Research in Toxicology
- Vol. 22 (1), 227-234
- https://doi.org/10.1021/tx8004168
Abstract
The major biophenols in olives and the crude extract and ethyl acetate fraction from olive mill waste were studied for their ability to counteract different stages of oxidative damage, that is, hydrogen peroxide, superoxide radical (SOR), and hydroxyl radical in vitro. Antiproliferative activity on colon cancer (HT-29) and gastric cancer (AGS) cell lines was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide bioassay. Emphasis was given to how the observed in vitro activity is controlled by the structural feature of biophenols and possible synergism and antagonism. While in some bioassays, for example, 2,2′-diphenyl-1-picrylhydrazyl, the nonphenolic moiety had minimal affect, it had a significant role in the SOR scavenging bioassay. Verbascoside was more active than caffeic acid or hydroxytyrosol evaluated individually or in equimolar mixtures in some bioassays. Mixtures of biophenols were more active than individual biophenols as antiproliferative agents. Overall, the mixture of hydroxytyrosol/caffeic acid and the biophenol extracts were more effective in protecting DNA from oxidative damage and inhibiting the growth of cancer cells.Keywords
This publication has 29 references indexed in Scilit:
- Potent antioxidant biophenols from olive mill wasteFood Chemistry, 2008
- Chemical screening of olive biophenol extracts by hyphenated liquid chromatographyAnalytica Chimica Acta, 2007
- Novel Secoiridoids with Antioxidant Activity from Australian Olive Mill WasteJournal of Agricultural and Food Chemistry, 2007
- Screening Methods for Antioxidants-A ReviewMini-Reviews in Medicinal Chemistry, 2006
- Bioactivity and Analysis of Biophenols Recovered from Olive Mill WasteJournal of Agricultural and Food Chemistry, 2005
- Olives and olive oil in cancer preventionEuropean Journal of Cancer Prevention, 2004
- Antioxidant and other biological activities of phenols from olives and olive oilMedicinal Research Reviews, 2001
- Methods for testing antioxidant activityThe Analyst, 2001
- The problems of using one-dimensional methods to evaluate multifunctional food and biological antioxidantsJournal of the Science of Food and Agriculture, 2000
- Aceite de oliva e inhibición de la oxidación de las lipoproteínas de baja densidad. Importancia de los compuestos fenólicosMedicina Clinica, 2000