The effectiveness of the combination of Moringa oleifera extract and propolis on Porphyromonas gingivalis biofilms compared to 0.7% tetracycline
Open Access
- 19 April 2021
- journal article
- Published by Universitas Airlangga in Dental Journal (Majalah Kedokteran Gigi)
- Vol. 54 (2), 63-67
- https://doi.org/10.20473/j.djmkg.v54.i2.p63-67
Abstract
Background: Periodontitis is an inflammatory disease that occurs in periodontal tissues. Porphyromonas gingivalis is also known as a bacterium commonly associated with the pathogenesis of periodontitis. Tetracycline is one of the antibiotics often used in periodontal tissue treatment. Propolis and Moringa oleifera are also known to have certain compounds assumed to be able to inhibit biofilm growth. Purpose: This study aims to understand the effectiveness of the combination of Moringa oleifera and propolis on porphyomonas gingivalis biofilms compared to 0.7% tetracycline. Methods: A biofilm inhibition activity test was performed using the broth micro dilution method. First, bacteria were prepared by making a suspension in brain heart infusion media and adjusting it to 0.5 McFarland I standard. Second, fifteen samples were divided into five groups; group K as control group (0.1% sodium carboxymethyl cellulose), T (0.7% tetracycline), and treatment groups with the combination of propolis and Moringa oleifera in various concentrations, such as P1(10%+20%), P2(10%+40%), and P3(10%+80%). Third, the result data obtained in the form of optical density (OD) was read by using an ELISA reader. Next, statistical analysis using analysis of the variance test was conducted (pResults: There was no significant difference between group T and group P1 (0.075). Nevertheless, there were significant differences between group T and group P2 as well as between group T and group P3 (0.00) (p=< 0.05). Conclusion: The combination of 10% propolis and 40% Moringa oleifera as well as the combination of 10% propolis and 80% Moringa oleifera have better antibacterial effectiveness against Porphyromonas gingivalis biofilm than 0.7% tetracycline.Keywords
This publication has 15 references indexed in Scilit:
- PENGARUH VARIASI PH TERHADAP KADAR FLAVONOID PADA EKSTRAKSI PROPOLIS DAN KARAKTERISTIKNYA SEBAGAI ANTIMIKROBAJURNAL KONVERSI, 2017
- Tetrasiklin HCL Gel 0,7% meningkatkan jumlah sel fbroblas dan mempertebal ligamen periodontal pada sulkus gingiva tikus yang mengalami periodontitisIntisari Sains Medis, 2017
- New approaches to combat Porphyromonas gingivalis biofilmsJournal of Oral Microbiology, 2017
- Comparison of the efficacy of subgingival irrigation with 2% povidone-iodine and tetracycline HCl in subjects with chronic moderate periodontitis: A clinico microbiological studyDental Research Journal, 2016
- Pharmaceuticals: Tetracyclines and periodontal diseaseBritish Dental Journal, 2015
- Bacterial Biofilms: Survival Mechanisms and Antibiotic ResistanceJournal of Bacteriology & Parasitology, 2014
- Bacterial interactions in dental biofilmVirulence, 2011
- Propolis research and the chemistry of plant productsNatural Product Reports, 2011
- Microtiter Dish Biofilm Formation AssayJournal of Visualized Experiments, 2011
- Biofilms: A microbial homeJournal of Indian Society of Periodontology, 2011