Tetrasiklin HCL Gel 0,7% meningkatkan jumlah sel fbroblas dan mempertebal ligamen periodontal pada sulkus gingiva tikus yang mengalami periodontitis

Abstract

Periodontal disease is an infectious disease, could be result loose of the teeth. This research is to prove that the applying 0.7% tetracycline hcl gel on rat gingival sulcus result higher number of fibroblasts and collagen synthesis which are marked by the thicker of rat periodontal ligament. The study was a randomized post test only control group design experimental study on 32 white male rats which are inducted by lipopolyssaccaride (LPS) for 8 days. And then rats divided into control group were given placebo, and treatment group were given 0.7% tetracycline HCL gel. On 10th days, the rats were euthanazided for taken mandibula alveolar bone tissue sampling and histological preparations were made by HE (Harris Hematoxylin-Eosin) staining. The data were analyzed using SPSS program processed using Shapiro Wilk for normality test and then continued using independent t-test. The result of this research is the mean number of fibroblasts at treatment group by administration of 0.7% tetracycline HCL gel (148.38±5.11 units) was higher than the control group (113.19±5.39 units). The mean of collagen synthesis was showed by the thickness of periodontal ligamen at group treated 0.7% tetracycline HCL gel (338.65±57.28 µm) was higher than the control group (225.50±45.29 µm). The mean number of fibroblast cell and thickness of periodontal ligament were statistically different (p<0.05). Thus, it is concluded that 0.7% tetracycline HCL gel enhance the number of fibroblasts cell and the thickness of periodontal ligament in rat with periodontal disease.