Field Effect Sensors for Nucleic Acid Detection: Recent Advances and Future Perspectives
Open Access
- 4 May 2015
- Vol. 15 (5), 10380-10398
- https://doi.org/10.3390/s150510380
Abstract
In the last decade the use of field-effect-based devices has become a basic structural element in a new generation of biosensors that allow label-free DNA analysis. In particular, ion sensitive field effect transistors (FET) are the basis for the development of radical new approaches for the specific detection and characterization of DNA due to FETs’ greater signal-to-noise ratio, fast measurement capabilities, and possibility to be included in portable instrumentation. Reliable molecular characterization of DNA and/or RNA is vital for disease diagnostics and to follow up alterations in gene expression profiles. FET biosensors may become a relevant tool for molecular diagnostics and at point-of-care. The development of these devices and strategies should be carefully designed, as biomolecular recognition and detection events must occur within the Debye length. This limitation is sometimes considered to be fundamental for FET devices and considerable efforts have been made to develop better architectures. Herein we review the use of field effect sensors for nucleic acid detection strategies—from production and functionalization to integration in molecular diagnostics platforms, with special focus on those that have made their way into the diagnostics lab.Keywords
This publication has 87 references indexed in Scilit:
- Enhanced Sensing of Nucleic Acids with Silicon Nanowire Field Effect Transistor BiosensorsNano Letters, 2012
- Detection beyond the Debye Screening Length in a High-Frequency Nanoelectronic BiosensorNano Letters, 2012
- ISFET Based Microsensors for Environmental MonitoringSensors, 2009
- Ion-Sensitive Field-Effect Transistor for Biological SensingSensors, 2009
- Sensitivity of field-effect biosensors to charge, pH, and ion concentration in a membrane modelJournal of Applied Physics, 2008
- Surface activation of thin silicon oxides by wet cleaning and silanizationThin Solid Films, 2006
- Enzyme biosensors based on ion-selective field-effect transistorsAnalytica Chimica Acta, 2006
- Optical properties and deposition rate of sputtered Ta2O5 films deposited by ion-beam oxidationThin Solid Films, 2005
- Atomic layer deposition of Al2O3, ZrO2, Ta2O5, and Nb2O5 based nanolayered dielectricsJournal of Non-Crystalline Solids, 2002
- Processing and characterisation of sol–gel deposited Ta2O5 and TiO2–Ta2O5 dielectric thin filmsSolid-State Electronics, 1999