Antioxidant and free radical scavenging activity of Spondias pinnata
Top Cited Papers
Open Access
- 9 December 2008
- journal article
- Published by Springer Science and Business Media LLC in BMC Complementary and Alternative Medicine
- Vol. 8 (1), 63
- https://doi.org/10.1186/1472-6882-8-63
Abstract
Background: Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally-occurring antioxidants of plant origin. The aim of the present study was to evaluate thein vitroantioxidant activities ofSpondias pinnatastem bark extract.Methods: A 70% methanol extract ofSpondias pinnatastem bark was studiedin vitrofor total antioxidant activity, for scavenging of hydroxyl radicals, superoxide anions, nitric oxide, hydrogen peroxide, peroxynitrite, singlet oxygen and hypochlorous acid, and for iron chelating capacity, reducing power, and phenolic and flavonoid contents.Results: The extract showed total antioxidant activity with a trolox equivalent antioxidant concentration (TEAC) value of 0.78 ± 0.02. The IC50values for scavenging of free radicals were 112.18 ± 3.27 μg/ml, 13.46 ± 0.66 μg/ml and 24.48 ± 2.31 μg/ml for hydroxyl, superoxide and nitric oxide, respectively. The IC50for hydrogen peroxide scavenging was 44.74 ± 25.61 mg/ml. For the peroxynitrite, singlet oxygen and hypochlorous acid scavenging activities the IC50values were 716.32 ± 32.25 μg/ml, 58.07 ± 5.36 μg/ml and 127.99 ± 6.26 μg/ml, respectively. The extract was found to be a potent iron chelator with IC50= 66.54 ± 0.84 μg/ml. The reducing power was increased with increasing amounts of extract. The plant extract (100 mg) yielded 91.47 ± 0.004 mg/ml gallic acid-equivalent phenolic content and 350.5 ± 0.004 mg/ml quercetin-equivalent flavonoid content.Conclusion: The present study provides evidence that a 70% methanol extract ofSpondias pinnatastem bark is a potential source of natural antioxidants.Keywords
This publication has 41 references indexed in Scilit:
- Nasturtium officinale reduces oxidative stress and enhances antioxidant capacity in hypercholesterolaemic ratsChemico-Biological Interactions, 2008
- Hypochlorous acid scavenging capacity of garlicPhytotherapy Research, 2007
- Optimisation of in vitro measurement of available iron from different fortificants in citric fruit juicesFood Chemistry, 2006
- Nordihydroguaiaretic acid is a potentin vitroscavenger of peroxynitrite, singlet oxygen, hydroxyl radical, superoxide anion and hypochlorous acid and preventsin vivoozone-induced tyrosine nitration in lungsFree Radical Research, 2006
- Hydrogen Peroxide in Human Urine: Implications for Antioxidant Defense and Redox RegulationBiochemical and Biophysical Research Communications, 1999
- The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicalsFood Chemistry, 1999
- Prospects for the Use of Antioxidant TherapiesDrugs, 1995
- The Reaction of no With SuperoxideFree Radical Research Communications, 1993
- Involvement of Singlet Oxygen in 5-Aminolevulinic Acid-Induced Photodynamic Damage of Cucumber (Cucumis sativus L.) ChloroplastsPlant Physiology, 1992
- STUDIES ON THE CHEMICAL CONSTITUENTS OFSPONDIAS PINNATAPlanta Medica, 1976