In vivo regeneration of small‐diameter (2 mm) arteries using a polymer scaffold
- 15 November 2005
- journal article
- Published by Wiley in The FASEB Journal
- Vol. 20 (1), 103-105
- https://doi.org/10.1096/fj.05-4802fje
Abstract
The difficulty of obtaining significant long-term patency and good wall mechanical strength in vivo has been a significant obstacle in achieving small-diameter vascular prostheses. The aim of the present study was to develop a prosthetic graft that could perform as a small-diameter vascular conduit. Tubular structures of hyaluronan (HYAFF-11 tubules, 2 mm diameter, 1 cm length) were grafted in the abdominal aorta of 30 rats as temporary absorbable guides to promote regeneration of vascular structures. Performance was assessed by histology, immunohistochemistry, and ultra-structural analysis. These experiments resulted in three novel findings: 1) complete endothelialization of the tube's luminal surface occurred; 2) sequential regeneration of vascular components led to complete vascular wall regeneration 15 days after surgery; and 3) the biomaterial used created the ideal environment for the delicate regeneration process during the critical initial phases, yet its biodegradability allowed for complete degradation of the construct four months after implantation, at which time, a new artery remained to connect the artery stumps. This study assesses the feasibility to create a completely biodegradable vascular regeneration guide in vivo, able to sequentially orchestrate vascular regeneration events needed for very small artery reconstruction.Keywords
This publication has 36 references indexed in Scilit:
- Artificial blood vessel: The Holy Grail of peripheral vascular surgeryJournal of Vascular Surgery, 2005
- Radial Artery Bypass Grafts Have an Increased Occurrence of Angiographically Severe Stenosis and Occlusion Compared With Left Internal Mammary Arteries and Saphenous Vein GraftsCirculation, 2004
- In Vivo Tissue-Engineered Small-Caliber Arterial Graft Prosthesis Consisting of Autologous Tissue (Biotube)Cell Transplantation, 2004
- Saphenous Vein Versus PTFE for Above-Knee Femoropopliteal Bypass. A Review of the LiteratureEuropean Journal of Vascular and Endovascular Surgery, 2004
- Creation of long-lasting blood vesselsNature, 2004
- Engineering conduits to resemble natural vascular tissueBiotechnology and Applied Biochemistry, 2004
- Recent Progress of Vascular Graft Engineering in JapanArtificial Organs, 2004
- In vivo engineering of blood vesselsFrontiers in Bioscience-Landmark, 2004
- Advances in vascular tissue engineeringCardiovascular Pathology, 2003
- Pore size, tissue ingrowth, and endothelialization of small-diameter microporous polyurethane vascular prosthesesBiomaterials, 2003