High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Data Collection and Instrument Calibration: Figure 1.
Open Access
- 1 October 2009
- journal article
- Published by Cold Spring Harbor Laboratory in Cold Spring Harbor Protocols
- Vol. 2009 (10), pdb.ip74
- https://doi.org/10.1101/pdb.ip74
Abstract
INTRODUCTION: Optical traps or “optical tweezers” have become an indispensable tool in understanding fundamental biological processes. Dual-trap optical tweezers with differential detection have the advantage that the instrument is decoupled from sources of environmental noise, thereby minimizing interference of signal and maximizing stability and spatial resolution. In this article, we describe methods of data detection and calibration for our dual-trap design.Keywords
This publication has 9 references indexed in Scilit:
- High-Resolution Dual-Trap Optical Tweezers with Differential Detection: An Introduction: Figure 1.Cold Spring Harbor Protocols, 2009
- High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Alignment of Instrument Components: Figure 1.Cold Spring Harbor Protocols, 2009
- High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Minimizing the Influence of Measurement NoiseCold Spring Harbor Protocols, 2009
- High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Instrument DesignCold Spring Harbor Protocols, 2009
- High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Managing Environmental Noise: Figure 1.Cold Spring Harbor Protocols, 2009
- Calibration of optical tweezers with positional detection in the back focal planeReview of Scientific Instruments, 2006
- Calibrating bead displacements in optical tweezers using acousto-optic deflectorsReview of Scientific Instruments, 2006
- Optical trappingReview of Scientific Instruments, 2004
- Power spectrum analysis for optical tweezersReview of Scientific Instruments, 2004