High-Resolution Dual-Trap Optical Tweezers with Differential Detection: An Introduction: Figure 1.
Open Access
- 1 October 2009
- journal article
- Published by Cold Spring Harbor Laboratory in Cold Spring Harbor Protocols
- Vol. 2009 (10), pdb.top60
- https://doi.org/10.1101/pdb.top60
Abstract
INTRODUCTION: Optical traps or “optical tweezers” have become an indispensable tool in understanding fundamental biological processes. The ability to manipulate and probe individual molecules or molecular complexes has led to a new, more refined understanding of the mechanical properties of the fundamental building blocks of the cell, and of the mechanism by which molecular machines function. The field has seen a steady stream of technological advances that have greatly refined the technique. One major effort has been in developing methods to resolve motions at the angstrom level--the fundamental length scale for many biological processes. This drive has only recently come to fruition with the advent of high-resolution optical trapping techniques that can now detect movements on the scale of a single base pair of DNA, 3.4 Å. Here we briefly review the basic concepts and components of optical traps and the single-molecule experiments in which they are used.Keywords
This publication has 13 references indexed in Scilit:
- High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Alignment of Instrument Components: Figure 1.Cold Spring Harbor Protocols, 2009
- High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Minimizing the Influence of Measurement NoiseCold Spring Harbor Protocols, 2009
- High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Data Collection and Instrument Calibration: Figure 1.Cold Spring Harbor Protocols, 2009
- High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Instrument DesignCold Spring Harbor Protocols, 2009
- High-Resolution Dual-Trap Optical Tweezers with Differential Detection: Managing Environmental Noise: Figure 1.Cold Spring Harbor Protocols, 2009
- Differential detection of dual traps improves the spatial resolution of optical tweezersProceedings of the National Academy of Sciences of the United States of America, 2006
- Backtracking by single RNA polymerase molecules observed at near-base-pair resolutionNature, 2003
- Ten years of tension: single-molecule DNA mechanicsNature, 2003
- Observation of a single-beam gradient force optical trap for dielectric particlesOptics Letters, 1986
- Acceleration and Trapping of Particles by Radiation PressurePhysical Review Letters, 1970