Isolation and Bacterial Expression of a Murine Alpha Leukocyte Interferon Gene
- 1 January 1984
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Interferon Research
- Vol. 4 (4), 635-643
- https://doi.org/10.1089/jir.1984.4.635
Abstract
A murine alpha interferon gene (MuIFN-αA) was isolated from a murine genomic DNA library in phage lambda. The gene was cloned and its sequence determined. It was expressed in Escherichia coli under the control of the trp promoter/operator which resulted in antiviral activity on mouse L-cells. MuIFN-αA exhibits 60% homology to HuIFN-αA at the amino acid level.Keywords
This publication has 19 references indexed in Scilit:
- Quantitative isolation of DNA restriction fragments from low-melting agarose by Elutip-d affinity chromatographyAnalytical Biochemistry, 1983
- The human interferons—From protein purification and sequence to cloning and expression in bacteria: Before, between, and beyondArchives of Biochemistry and Biophysics, 1983
- The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplicationGene, 1981
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesisTetrahedron Letters, 1981
- Human leukocyte interferon produced by E. coli is biologically activeNature, 1980
- Mouse Interferons: Amino Terminal Amino Acid Sequences of Various SpeciesScience, 1980
- The isolation and characterization of linked δ- and β-globin genes from a cloned library of human DNACell, 1978
- Construction and characterization of new cloning vehicles III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA moleculesGene, 1978
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresisBiochemistry, 1973