Human leukocyte interferon produced by E. coli is biologically active
- 1 October 1980
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 287 (5781), 411-416
- https://doi.org/10.1038/287411a0
Abstract
A human leukocyte interferon cDNA was enzymatically synthesized, inserted into the vector pBR322, and cloned in Escherichia coli. The DNA sequence codes for a 23-amino acid signal peptide followed by an interferon polypeptide of 165 amino acids. An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 108 units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus infection.This publication has 29 references indexed in Scilit:
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