Co-localization of the nerve growth factor precursor protein and mRNA in the mouse submandibular gland
- 15 February 1988
- journal article
- Published by Elsevier BV in Neuroscience Letters
- Vol. 85 (1), 19-23
- https://doi.org/10.1016/0304-3940(88)90421-1
Abstract
Indirect immunofluorescence on frozen sections of the mouse submandibular gland (MSG) and affinitypurified sera directed against synthetic peptides that reproduce sequences of the nerve growth factor (NGF) precursor protein permitted its localization in the basal parts of the cells forming the secretory tubules. In situ hybridization experiments employing 35S-labeled NGF cDNA probe localized the NGF mRNA in the same region. Conversion of proNGF to mature NGF results in an altered localization of the cleaved peptide throughout the cytoplasm of the tubular cells with a preferential concentration at their apical pole.Keywords
This publication has 7 references indexed in Scilit:
- Identification and quantification of mRNA for nerve growth factor in histological preparationsNeuroscience Letters, 1986
- Precursors of the nerve growth factor γ subunit and renin bind to microtubulesEuropean Journal of Biochemistry, 1984
- Synthesis and partial maturation of the α‐ and γ‐subunits of the mouse submaxillary gland nerve growth factor in Xenopus laevis oocytesFEBS Letters, 1984
- Immunocytochemical localization of nerve growth factor: effects of fixation.Journal of Histochemistry & Cytochemistry, 1984
- Human β-nerve growth factor gene sequence highly homologous to that of mouseNature, 1983
- Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factorNature, 1983
- Distribution of nerve growth factor in the submandibular gland of the male and female mouseCell and tissue research, 1981