High levels of virus replication and an intense inflammatory response contribute to the severe pathology in lymphoid tissues caused by Newcastle disease virus genotype VIId
- 12 December 2014
- journal article
- research article
- Published by Springer Science and Business Media LLC in Archiv für die gesamte Virusforschung
- Vol. 160 (3), 639-648
- https://doi.org/10.1007/s00705-014-2301-2
Abstract
Some strains of Newcastle disease virus (NDV) genotype VIId cause more-severe tissue damage in lymphoid organs compared to other virulent strains. In this study, we aim to define the mechanism of this distinct pathological manifestation of genotype VII viruses. Pathology, virus replication, and the innate immune response in lymphoid tissues of chickens infected with two genotype VIId NDV strains (JS5/05 and JS3/05), genotype IX NDV F48E8 and genotype IV NDV Herts/33, were compared. Histopathologic examination showed that JS5/05 and JS3/05 produced more-severe lesions in the spleen and thymus, but these four virulent strains caused comparable mild lesions in the bursa. In addition, JS3/05 and JS5/05 replicated at significantly higher levels in the lymphatic organs than F48E8 and Herts/33. A microarray assay performed on the spleens of chickens infected with JS5/05 or Herts/33 revealed that JS5/05 elicited a more potent inflammatory response by increasing the number and expression levels of activated genes. Moreover, cytokine gene expression profiling showed that JS5/05 and JS3/05 induced a stronger cytokine response in lymphoid tissues compared to F48E8 and Herts/33. Taken together, our results indicate that the severe pathology in immune organs caused by genotype VIId NDV strains is associated with high levels of virus replication and an intense inflammatory response.Keywords
This publication has 22 references indexed in Scilit:
- Strong innate immune response and cell death in chicken splenocytes infected with genotype VIId Newcastle disease virusVirology Journal, 2012
- Lack of detection of host associated differences in Newcastle disease viruses of genotype VIId isolated from chickens and geeseVirology Journal, 2012
- Generation of a Genotype VII Newcastle Disease Virus Vaccine Candidate with High Yield in Embryonated Chicken EggsAvian Diseases, 2011
- In vivo transcriptional cytokine responses and association with clinical and pathological outcomes in chickens infected with different Newcastle disease virus isolates using formalin-fixed paraffin-embedded samplesVeterinary Immunology and Immunopathology, 2011
- Genetic diversity of Newcastle disease viruses isolated from domestic poultry species in Eastern China during 2005–2008Archiv für die gesamte Virusforschung, 2010
- Clinicopathological Characterization in Poultry of Three Strains of Newcastle Disease Virus Isolated From Recent OutbreaksVeterinary Pathology, 2010
- Association of Increased Pathogenicity of Asian H5N1 Highly Pathogenic Avian Influenza Viruses in Chickens with Highly Efficient Viral Replication Accompanied by Early Destruction of Innate Immune ResponsesJournal of Virology, 2009
- A vaccine candidate of attenuated genotype VII Newcastle disease virus generated by reverse geneticsVaccine, 2009
- Pathotypical Characterization and Molecular Epidemiology of Newcastle Disease Virus Isolates from Different Hosts in China from 1996 to 2005Journal of Clinical Microbiology, 2008
- Experimental Pathogenesis for Chickens, Turkeys, and Pigeons of Exotic Newcastle Disease Virus from an Outbreak in California during 2002-2003Veterinary Pathology, 2006