Border-cell migration requires integration of spatial and temporal signals by the BTB protein Abrupt
Open Access
- 6 April 2009
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 11 (5), 569-579
- https://doi.org/10.1038/ncb1863
Abstract
How temporal signals from the steroid hormone ecdysone are integrated with JAK/STAT-mediated spatial control of Drosophila border-cell migration has been unclear. JAK/STAT represses Abrupt, which in turn attenuates ecdysone signalling by interacting with the ecdysone receptor coactivator Taiman. During development, elaborate patterns of cell differentiation and movement must occur in the correct locations and at the proper times. Developmental timing has been studied less than spatial pattern formation, and the mechanisms integrating the two are poorly understood. Border-cell migration in the Drosophila ovary occurs specifically at stage 9. Timing of the migration is regulated by the steroid hormone ecdysone, whereas spatial patterning of the migratory population requires localized activity of the JAK–STAT pathway. Ecdysone signalling is patterned spatially as well as temporally, although the mechanisms are not well understood. In stage 9 egg chambers, ecdysone signalling is highest in anterior follicle cells including the border cells. We identify the gene abrupt as a repressor of ecdysone signalling and border-cell migration. Abrupt protein is normally lost from border-cell nuclei during stage 9, in response to JAK–STAT activity. This contributes to the spatial pattern of the ecdysone response. Abrupt attenuates ecdysone signalling by means of a direct interaction with the basic helix–loop–helix (bHLH) domain of the P160 ecdysone receptor coactivator Taiman (Tai). Taken together, these findings provide a molecular mechanism by which spatial and temporal cues are integrated.Keywords
This publication has 59 references indexed in Scilit:
- Temporal Regulation of Metamorphic Processes in Drosophila by the let-7 and miR-125 Heterochronic MicroRNAsCurrent Biology, 2008
- A matter of timing: microRNA-controlled temporal identities in worms and fliesGenes & Development, 2008
- Drosophila let-7 microRNA is required for remodeling of the neuromusculature during metamorphosisGenes & Development, 2008
- Feedback Inhibition of JAK/STAT Signaling by Apontic Is Required to Limit an Invasive Cell PopulationDevelopmental Cell, 2008
- Understanding morphogen gradients: a problem of dispersion and containmentCurrent Opinion in Genetics & Development, 2007
- The Genetic Basis for the Timing of Human PubertyJournal of Neuroendocrinology, 2007
- Heterochronic Genes and the Nature of Developmental TimeCurrent Biology, 2007
- Rap-GEF Signaling Controls Stem Cell Anchoring to Their Niche through Regulating DE-Cadherin-Mediated Cell Adhesion in the Drosophila TestisDevelopmental Cell, 2006
- Development of Morphological Diversity of Dendrites in Drosophila by the BTB-Zinc Finger Protein AbruptNeuron, 2004
- Border-cell migration: the race is onNature Reviews Molecular Cell Biology, 2003