Subcellular localization and mobilization of carcinoembryonic antigen‐related cell adhesion molecule 8 in human neutrophils
- 27 April 2004
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 125 (5), 666-673
- https://doi.org/10.1111/j.1365-2141.2004.04963.x
Abstract
The subcellular localization and mobilization of carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8) was investigated quantitatively in human neutrophils. In resting neutrophils the majority of CEACAM8 was present in the secondary granules, and a small amount of CEACAM8 was present in a light membrane fraction. Stimulation of the neutrophils with phorbol 12-myristate 13-acetate caused a dramatic increase in the content of CEACAM8 in the light membrane fraction, suggesting a translocation of CEACAM8 to the plasma membrane from intracellular pools. The cellular content of CEACAM8 in the neutrophils was estimated to be 82.4 +/- 8.9 ng/10(6) cells (mean +/- SE, n = 10). Administration of granulocyte colony-stimulating factor (G-CSF) to healthy individuals resulted in an increased content of CEACAM8 in neutrophils on day 1, which decreased on day 4. However, the content of CEACAM8 in the light membrane fraction was increased on day 4, possibly due to the stimulation by induced secondary cytokines, such as tumour necrosis factor-alpha (TNF-alpha). This study establishes the secondary granules as the major intracellular pools of CEACAM8 in human neutrophils, from which it may translocate to the plasma membranes upon stimulation of the cells. The translocation of CEACAM8 seen in vivo after G-CSF administration is probably indirect and caused by cytokines such as TNF-alpha.Keywords
This publication has 34 references indexed in Scilit:
- The effect of granulocyte colony‐stimulating factor (G‐CSF) on the degranulation of secondary granule proteins from human neutrophils in vivo may be indirectBritish Journal of Haematology, 1996
- Analysis of Heterophilic Cell Adhesion Mediated by CD66b and CD66c Using Their Soluble Recombinant ProteinsBiochemical and Biophysical Research Communications, 1996
- Serum levels of granulocyte‐colony stimulating factor (G‐CSF) in bacterial and viral infections, and in atypical pneumoniaBritish Journal of Haematology, 1994
- Granules and vesicles of human neutrophils. The role of endomembranes as source of plasma membrane proteinsEuropean Journal of Haematology, 1993
- Granulocyte Colony-Stimulating Factor and Granulocyte-Macrophage Colony-Stimulating FactorNew England Journal of Medicine, 1992
- Marrow proliferation and the appearance of giant neutrophils in response to recombinant human granulocyte colony stimulating factor (rhG-CSF)British Journal of Haematology, 1992
- Regulation of mRNA levels of alkaline phosphatase gene in neutrophilic granulocytes by granulocyte colony-stimulating factor and retinoic acidEuropean Journal of Haematology, 1991
- Carcinoembryonic antigen, a human tumor marker, functions as an intercellular adhesion moleculeCell, 1989
- Tissue Destruction by NeutrophilsNew England Journal of Medicine, 1989
- Serum Myeloperoxidase and Lactoferrin in NeutropeniaScandinavian Journal of Haematology, 1977