Near Infrared Imaging and Photothermal Ablation of Vascular Inflammation Using Single‐Walled Carbon Nanotubes
Open Access
- 29 November 2012
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of the American Heart Association
- Vol. 1 (6), e002568
- https://doi.org/10.1161/jaha.112.002568
Abstract
Background: Macrophages are critical contributors to atherosclerosis. Single‐walled carbon nanotubes ( SWNT s) show promising properties for cellular imaging and thermal therapy, which may have application to vascular macrophages. Methods and Results: In vitro uptake and photothermal destruction of mouse macrophage cells ( RAW 264.7) were performed with SWNT s (14.7 nmol/L) exposed to an 808‐nm light source. SWNT s were taken up by 94±6% of macrophages, and light exposure induced 93±3% cell death. In vivo vascular macrophage uptake and ablation were then investigated in carotid‐ligated FVB mice (n=33) after induction of hyperlipidemia and diabetes. Two weeks postligation, near‐infrared fluorescence ( NIRF ) carotid imaging (n=12) was performed with SWNT ‐Cy5.5 (8 nmol of Cy5.5) given via the tail vein. Photothermal heating and macrophage apoptosis were evaluated on freshly excised carotid arteries (n=21). NIRF of SWNT s showed higher signal intensity in ligated carotids compared with sham, confirmed by both in situ and ex vivo NIRF imaging ( P <0.05, ligation versus sham). Immunofluorescence staining showed colocalization of SWNT ‐Cy5.5 and macrophages in atherosclerotic lesions. Light (808 nm) exposure of freshly excised carotids showed heating and induction of macrophage apoptosis in ligated left carotid arteries with SWNT s, but not in control groups without SWNT s or without light exposure. Conclusions: Carbon nanotubes accumulate in atherosclerotic macrophages in vivo and provide a multifunctional platform for imaging and photothermal therapy of vascular inflammation.Keywords
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