Genetic Diversity of Potassium Ion Channel Proteins Encoded by Chloroviruses That Infect Chlorella heliozoae
Open Access
- 23 June 2020
- Vol. 12 (6), 678
- https://doi.org/10.3390/v12060678
Abstract
Chloroviruses are large, plaque-forming, dsDNA viruses that infect chlorella-like green algae that live in a symbiotic relationship with protists. Chloroviruses have genomes from 290 to 370 kb, and they encode as many as 400 proteins. One interesting feature of chloroviruses is that they encode a potassium ion (K+) channel protein named Kcv. The Kcv protein encoded by SAG chlorovirus ATCV-1 is one of the smallest known functional K+ channel proteins consisting of 82 amino acids. The KcvATCV-1 protein has similarities to the family of two transmembrane domain K+ channel proteins; it consists of two transmembrane α-helixes with a pore region in the middle, making it an ideal model for studying K+ channels. To assess their genetic diversity, kcv genes were sequenced from 103 geographically distinct SAG chlorovirus isolates. Of the 103 kcv genes, there were 42 unique DNA sequences that translated into 26 new Kcv channels. The new predicted Kcv proteins differed from KcvATCV-1 by 1 to 55 amino acids. The most conserved region of the Kcv protein was the filter, the turret and the pore helix were fairly well conserved, and the outer and the inner transmembrane domains of the protein were the most variable. Two of the new predicted channels were shown to be functional K+ channels.Keywords
Funding Information
- Office of Experimental Program to Stimulate Competitive Research (1736030)
- European Research Council (Advanced Grant 495 (AdG) n. 695078 noMAGIC)
This publication has 22 references indexed in Scilit:
- Towards defining the chloroviruses: a genomic journey through a genus of large DNA virusesBMC Genomics, 2013
- Initial Events Associated with Virus PBCV-1 Infection of Chlorella NC64AProgress in Botany, 2009
- An icosahedral algal virus has a complex unique vertex decorated by a spikeProceedings of the National Academy of Sciences of the United States of America, 2009
- Chlorella virus ATCV-1 encodes a functional potassium channel of 82 amino acidsBiochemical Journal, 2009
- Chlorovirus-Mediated Membrane Depolarization ofChlorellaAlters Secondary Active Transport of SolutesJournal of Virology, 2008
- Chlorella viruses evoke a rapid release of K+ from host cells during the early phase of infectionVirology, 2008
- Chlorella virus MT325 encodes water and potassium channels that interact synergisticallyProceedings of the National Academy of Sciences of the United States of America, 2006
- A Potassium Channel Protein Encoded by Chlorella Virus PBCV-1Science, 2000
- Mutual Exclusion Occurs in a Chlorella-like Green Alga Inoculated with Two VirusesJournal of General Virology, 1989
- Virus Infection of Culturable Chlorella -Like Algae and Development of a Plaque AssayScience, 1983