Initial Events Associated with Virus PBCV-1 Infection of Chlorella NC64A

Abstract

Chlorella viruses (or chloroviruses) are very large, plaque-forming viruses. The viruses are multilayered structures containing a large double-stranded DNA genome, a lipid bilayered membrane, and an outer icosahedral capsid shell. The viruses replicate in certain isolates of the coccal green alga, Chlorella. Sequence analysis of the 330-kbp genome of Paramecium bursaria Chlorella virus 1 (PBCV-1), the prototype of the virus family Phycodnaviridae, reveals + channel. This chapter focuses on the initial steps in virus infection and provides a plausible role for the function of the viral K⁺ channel in lowering the turgor pressure of the host. This step appears to be a prerequisite for delivery of the viral genome into the host.