The Oct4 homologue PouV and Nanog regulate pluripotency in chicken embryonic stem cells
- 1 October 2007
- journal article
- Published by The Company of Biologists in Development
- Vol. 134 (19), 3549-3563
- https://doi.org/10.1242/dev.006569
Abstract
Embryonic stem cells (ESC) have been isolated from pregastrulation mammalian embryos. The maintenance of their pluripotency and ability to self-renew has been shown to be governed by the transcription factors Oct4(Pou5f1) and Nanog. Oct4 appears to control cell-fate decisions of ESC in vitro and the choice between embryonic and trophectoderm cell fates in vivo. In non-mammalian vertebrates, the existence and functions of these factors are still under debate, although the identification of the zebrafish pou2 (spg; pou5f1) and Xenopus Pou91(XlPou91) genes, which have important roles in maintaining uncommitted putative stem cell populations during early development, has suggested that these factors have common functions in all vertebrates. Using chicken ESC (cESC), which display similar properties of pluripotency and long-term self-renewal to mammalian ESC, we demonstrated the existence of an avian homologue of Oct4 that we call chicken PouV(cPouV). We established that cPouV and the chicken Nanog gene are required for the maintenance of pluripotency and self-renewal of cESC. These findings show that the mechanisms by which Oct4 and Nanog regulate pluripotency and self-renewal are not exclusive to mammals.Keywords
This publication has 58 references indexed in Scilit:
- The Caudal-Related Protein Cdx2 Promotes Trophoblast Differentiation of Mouse Embryonic Stem CellsThe International Journal of Cell Cloning, 2006
- Orphan Nuclear Receptor GCNF Is Required for the Repression of Pluripotency Genes during Retinoic Acid-Induced Embryonic Stem Cell DifferentiationMolecular and Cellular Biology, 2005
- Oct-3/4 and Sox2 Regulate Oct-3/4 Gene in Embryonic Stem CellsJournal of Biological Chemistry, 2005
- Self-renewal of teratocarcinoma and embryonic stem cellsOncogene, 2004
- Oct-4 Knockdown Induces Similar Patterns of Endoderm and Trophoblast Differentiation Markers in Human and Mouse Embryonic Stem CellsThe International Journal of Cell Cloning, 2004
- Zebrafish pou5f1/pou2, Homolog of Mammalian Oct4, Functions in the Endoderm Specification CascadeCurrent Biology, 2004
- Fbx15 Is a Novel Target of Oct3/4 but Is Dispensable for Embryonic Stem Cell Self-Renewal and Mouse DevelopmentMolecular and Cellular Biology, 2003
- Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complexNucleic Acids Research, 2002
- Regulation of Cre Recombinase Activity by Mutated Estrogen Receptor Ligand-Binding DomainsBiochemical and Biophysical Research Communications, 1997
- Cases in which Parsimony or Compatibility Methods Will be Positively MisleadingSystematic Zoology, 1978