Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complex
- 15 July 2002
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 30 (14), 3202-3213
- https://doi.org/10.1093/nar/gkf435
Abstract
Sox-2 is a transcriptional cofactor expressed in embryonic stem (ES) cells as well as in neuronal cells. It has been demonstrated that Sox-2 plays an important role in supporting gene expression in ES cells, especially by forming a complex with embryonic Octamer factor, Oct-3/4. Here, we have analyzed the regulatory regions of the Sox-2 gene and identified two enhancers which stimulate transcription in ES cells as well as in embryonal carcinoma cells. These regulatory regions, which we termed Sox regulatory regions (SRR) 1 and 2, exert their function specifically when cells are in an undifferentiated state. Interestingly, like the regulatory elements of FGF-4 and UTF1 genes, combinatorial action of Octamer and Sox-2 binding sites support the SRR2 activity. However, biochemical analyses reveal that, due to the unique sequence and/or its organization, the SRR2 bears distinct characteristics from those of FGF-4 and UTF1 regulatory elements. That is, unlike the FGF-4 gene enhancer, the SRR2 precludes the binding of the Oct-1-Sox-2 complex. The difference between the SRR2 and UTF1 regulatory element is in the ability of SRR2 to recruit the Oct-6-Sox-2 complex as well as the Oct-3/4-Sox-2 complex. Co-transfection analyses confirm that both complexes are able to stimulate transcription through the SRR2 element.This publication has 44 references indexed in Scilit:
- TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasmGenes & Development, 2001
- Pax6 and SOX2 form a co-DNA-binding partner complex that regulates initiation of lens developmentGenes & Development, 2001
- Coevolution of HMG domains and homeodomains and the generation of transcriptional regulation by Sox/POU complexesJournal of Cellular Physiology, 2001
- Flk1-positive cells derived from embryonic stem cells serve as vascular progenitorsNature, 2000
- Modulation of the Activity of Multiple Transcriptional Activation Domains by the DNA Binding Domains Mediates the Synergistic Action of Sox2 and Oct-3 on the Fibroblast Growth Factor-4EnhancerPublished by Elsevier BV ,2000
- Sox2 regulatory sequences direct expression of a (beta)-geo transgene to telencephalic neural stem cells and precursors of the mouse embryo, revealing regionalization of gene expression in CNS stem cells.2000
- Isolation, characterization, and differential expression of the murine Sox-2 promoterGene, 2000
- Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cellsNature Genetics, 2000
- Synergy of SF1 and RAR in Activation of Oct-3/4PromoterPublished by Elsevier BV ,2000
- Comparative expression of the mouse Sox1, Sox2 and Sox3 genes from pre-gastrulation to early somite stagesMechanisms of Development, 1999