Chemical Compositions, Chromatographic Fingerprints and Antioxidant Activities of Andrographis Herba
Open Access
- 10 November 2014
- Vol. 19 (11), 18332-18350
- https://doi.org/10.3390/molecules191118332
Abstract
This paper describes the development of an HPLC-UV-MS method for quantitative determination of andrographolide and dehydroandrographolide in Andrographis Herba and establishment of its chromatographic fingerprint. The method was validated for linearity, limit of detection and quantification, inter- and intra-day precisions, repeatability, stability and recovery. All the validation results of quantitative determination and fingerprinting methods were satisfactory. The developed method was then applied to assay the contents of andrographolide and dehydroandrographolide and to acquire the fingerprints of all the collected Andrographis Herba samples. Furthermore, similarity analysis and principal component analysis were used to reveal the similarities and differences between the samples on the basis of the characteristic peaks. More importantly, the DPPH free radical-scavenging and ferric reducing capacities of the Andrographis Herba samples were assayed. By bivariate correlation analysis, we found that six compounds are positively correlated to DPPH free radical scavenging and ferric reducing capacities, and four compounds are negatively correlated to DPPH free radical scavenging and ferric reducing capacities.This publication has 31 references indexed in Scilit:
- Qualitative and Quantitative Analysis of Andrographis paniculata by Rapid Resolution Liquid Chromatography/Time-of-Flight Mass SpectrometryMolecules, 2013
- Quality assessment on Polygoni Multiflori Caulis using HPLC/UV/MS combined with principle component analysisChemistry Central Journal, 2013
- Changes in the contents of four active diterpenoids at different growth stages in Andrographis paniculata (Burm.f.) Nees (Chuanxinlian)Chinese Medicine, 2013
- AP-1/IRF-3 Targeted Anti-Inflammatory Activity of Andrographolide Isolated fromAndrographis paniculataEvidence-Based Complementary and Alternative Medicine, 2013
- Are acupoints specific for diseases? A systematic review of the randomized controlled trials with sham acupuncture controlsChinese Medicine, 2010
- Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)Chinese Medicine, 2010
- Quality control of medicinal herbs Fructus gardeniae, Common Andrographis Herb and their preparations for their active constituents by high-performance liquid chromatography–photodiode array detection–electrospray mass spectrometryTalanta, 2008
- High-Throughput Relative DPPH Radical Scavenging Capacity AssayJournal of Agricultural and Food Chemistry, 2006
- Antioxidant and Anti-Inflammatory Activities of the PlantAndrographis PaniculataNeesImmunopharmacology and Immunotoxicology, 2006
- Inhibition of proprotein convertases-1, -7 and furin by diterpines of Andrographis paniculata and their succinoyl estersBiochemical Journal, 1999