The narK gene product participates in nitrate transport induced in Escherichia coli nitrate‐respiring cells
- 1 July 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 252 (1-2), 139-143
- https://doi.org/10.1016/0014-5793(89)80906-8
Abstract
The nucleotide sequence of the Escherichia coli narK gene, which is located in the upstream region of the narCHJI operon, was determined. The narK gene encodes a very hydrophobic protein with 463 amino acid residues (M r 49 693). A narK deletion mutant, under conditions for the induction of nitrate respiration, was unable to perform nitrate transport. Loss of transport activity was recovered by transforming the mutant with a narK + plasmid. Thus, we conclude that the narK gene encodes a transmembrane protein participating in nitrate transport. In the narK promoter region, we defined a unique sequence that we designate as a ‘nitrate box’, functioning as a putative NarL-binding site, in addition to the consensus sequence of the ‘anaero-box’.Keywords
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