Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
- 1 July 1983
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 80 (13), 3963-3965
- https://doi.org/10.1073/pnas.80.13.3963
Abstract
Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. A rapid way to pour a buffer concentration gradient gel was developed that, by altering the vertical band separation on an autoradiograph, allows more sequence to be obtained from a gel. The use of deoxyadenosine 5''-(.alpha.-[35S]thio)triphosphate as the label incorporated in dideoxynucleotide sequence reactions increases the sharpness of the bands on an autoradiography and so increases the resolution achieved.This publication has 10 references indexed in Scilit:
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