Interleukin‐10‐secreting Peyer's patch cells are responsible for active suppression in low‐dose oral tolerance
- 1 August 2001
- journal article
- research article
- Published by Wiley in Immunology
- Vol. 103 (4), 458-464
- https://doi.org/10.1046/j.1365-2567.2001.01265.x
Abstract
We demonstrate the induction of antigen-specific interleukin-10 (IL-10)-secreting cells in murine Peyer's patches (PPs) after low-dose β-lactoglobulin (BLG) feeding. In addition, we show that PP cells can inhibit the T-cell proliferative response in vitro as well as T-cell-mediated inflammation in vivo. The active suppression mediated by these regulatory cells was seen only within a narrow range of antigen dosage (feeding), with the most prominent effect at 5 × 1 mg BLG. On either side of this range, T-helper 1-like cytokine responses were observed when PP cells were stimulated with antigen in vitro. This result correlated with reduced production of regulatory cytokines as well as reduced activity of bystander suppression. We found that changes in IL-10 production correlated inversely with changes in interferon-γ production. Inhibitory effects mediated by CD4+ PP cells were partially neutralized by antibodies to IL-10 and transforming growth factor-β. Interestingly, the generation of such regulatory cells after low-dose BLG feeding exhibited organ dependence. Among spleen, lymph node and PP cells derived from orally tolerized mice, PP cells were the most effective in promoting bystander suppression in the presence of BLG, indicating the significance of PPs as an inductive site for antigen-specific regulatory cells upon induction of low-dose oral tolerance. Moreover, PP cells from mice fed 5 × 1 mg BLG were shown to suppress a BLG-specific delayed-type hypersensitivity response induced in footpads, suggesting that IL-10-secreting PP cells regulate systemic inflammation.Keywords
This publication has 28 references indexed in Scilit:
- Diversity in the contribution of interleukin‐10 to T‐cell‐mediated immune regulationImmunological Reviews, 2008
- IL-10: a potential therapy for allergic inflammation?Immunology Today, 1997
- DiscussionImmunology Today, 1997
- INDUCTION OF TH1 AND TH2 CD4+T CELL RESPONSES:The Alternative ApproachesAnnual Review of Immunology, 1997
- Regulatory T Cell Clones Induced by Oral Tolerance: Suppression of Autoimmune EncephalomyelitisScience, 1994
- Oral Tolerance: Immunologic Mechanisms and Treatment of Animal and Human Organ-Specific Autoimmune Diseases by Oral Administration of AutoantigensAnnual Review of Immunology, 1994
- Interleukin-10Annual Review of Immunology, 1993
- Interleukin-10Annual Review of Immunology, 1993
- Preferential Suppression of Delayed-type Hypersensitivity by L-156,602, a C5a Receptor AntagonistBioscience, Biotechnology, and Biochemistry, 1992
- The regulation of immune responses to dietary protein antigensImmunology Today, 1987