NosX function connects to nitrous oxide (N2O) reduction by affecting the CuZ center of NosZ and its activity in vivo
- 28 July 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (21), 4605-4609
- https://doi.org/10.1016/j.febslet.2005.07.023
Abstract
The effect of loss of the 34-kDa periplasmic NosX protein on the properties of N 2 O reductase was investigated with an N 2 O-respiration negative, double mutant of the paralogous genes nosX and nirX of Paracoccus denitrificans . In spite of absence of whole-cell N 2 O-reducing activity, the purified reductase was catalytically active, which attributes NosX a physiological role in sustaining the reaction cycle. N 2 O reductase exhibited the spectroscopic features of Cu A and the redox-inert, paramagnetic state, Cu Z ∗ , of the catalytic center. Cu Z ∗ , hitherto considered the result of spontaneous reaction of the reductase with dioxygen, attains cellular significance.This publication has 28 references indexed in Scilit:
- How does nitrous oxide reductase interact with its electron donors?—A docking studyProteins, 2005
- Functional Domains of NosR, a Novel Transmembrane Iron-Sulfur Flavoprotein Necessary for Nitrous Oxide RespirationJournal of Bacteriology, 2005
- Reductively Activated Nitrous Oxide Reductase Reacts Directly with SubstrateJournal of the American Chemical Society, 2004
- Requirements for Cu A and Cu-S Center Assembly of Nitrous Oxide Reductase Deduced from Complete Periplasmic Enzyme Maturation in the Nondenitrifier Pseudomonas putidaJournal of Bacteriology, 2003
- Role of the Tat Transport System in Nitrous Oxide Reductase Translocation and Cytochrome cd 1 Biosynthesis in Pseudomonas stutzeriJournal of Bacteriology, 2001
- A new nos gene downstream from nosDFY is essential for dissimilatory reduction of nitrous oxide by Rhizobium (Sinorhizobium) melilotiMicrobiology, 1997
- Purification and characterization of a nitrous oxide reductase from Thiosphaera pantotrophaJBIC Journal of Biological Inorganic Chemistry, 1993
- Nitrous oxide reductase from Pseudomonas stutzeriJBIC Journal of Biological Inorganic Chemistry, 1989
- The cupric site in nitrous oxide reductase contains a mixed‐valence [Cu(II),Cu(I)] binuclear center: A multifrequency electron paramagnetic resonance investigationFEBS Letters, 1988
- Nitrous oxide reductase from denitrifyingJBIC Journal of Biological Inorganic Chemistry, 1985