Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion
Top Cited Papers
- 27 February 2017
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Biotechnology
- Vol. 35 (5), 438-440
- https://doi.org/10.1038/nbt.3811
Abstract
Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR–Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protospacer in both protoplasts and regenerated rice, wheat and maize plants at frequencies of up to 43.48%.This publication has 25 references indexed in Scilit:
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