Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells
- 10 October 2016
- journal article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 13 (12), 1029-1035
- https://doi.org/10.1038/nmeth.4027
Abstract
Fusing a cytidine deaminase to dCas9 allows for a forward genetic screen in mammalian cells that identifies mutations conferring drug resistance.Keywords
This publication has 44 references indexed in Scilit:
- Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatorial librariesProceedings of the National Academy of Sciences, 2013
- Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene ExpressionCell, 2013
- A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial ImmunityScience, 2012
- A system for the continuous directed evolution of biomoleculesNature, 2011
- The Biochemistry of Somatic HypermutationAnnual Review of Immunology, 2008
- Mutational pattern and frequency of induced nucleotide changes in mouse ENU mutagenesisBMC Molecular Biology, 2007
- Intricate targeting of immunoglobulin somatic hypermutation maximizes the efficiency of affinity maturationThe Journal of Experimental Medicine, 2005
- R-loops at immunoglobulin class switch regions in the chromosomes of stimulated B cellsNature Immunology, 2003
- Clinical Resistance to STI-571 Cancer Therapy Caused by BCR-ABL Gene Mutation or AmplificationScience, 2001
- Crystal structure of human uracil-DNA glycosylase in complex with a protein inhibitor: Protein mimicry of DNACell, 1995