Ignatzschineria indica sp. nov. and Ignatzschineria ureiclastica sp. nov., isolated from adult flesh flies (Diptera: Sarcophagidae)

Abstract

Two Gram-negative-staining, aerobic, non-motile, rod-shaped bacteria, designated strains FFA1^T and FFA3^T, and belonging to the class Gammaproteobacteria were isolated from the gastrointestinal tract of adult flesh flies (Diptera: Sarcophagidae). Phylogenetic analysis of 16S rRNA gene sequence data placed these two strains within the genus Ignatzschineria with similarities of 98.6 % (FFA1^T) and 99.35 % (FFA3^T) to Ignatzschineria larvae L1/68^T. The level of gene sequence similarity between strains FFA1^T and FFA3^T was 99 %, 97.15 % and 78.1 % based on the 16S rRNA, 23S rRNA and gyrB gene sequences, respectively. Strains FFA1^T and FFA3^T shared 24 % DNA–DNA relatedness. DNA–DNA hybridization revealed a very low level of relatedness between the novel strains (22 % for strain FFA1^T and 44 % for strain FFA3^T) and I. larvae L1/68^T genomic DNA. The respiratory quinone was Q-8 in both novel strains. The DNA G+C contents were 41.1 mol% and 40.1 mol% for strains FFA1^T and FFA3^T, respectively. The cell membrane of both strains consisted of phosphatidylglycerol, phosphatidylethanolamine, phospholipids and aminophospholipid. The major fatty acids for both strains were C_16 : 0, summed feature 8 (C_18 : 1ω7c and/or C_18 : 1ω6c), CyC_19 : 0ω8c and C_14 : 0. The results of DNA–DNA hybridization between the two new strains and I. larvae L1/68^T, in combination with phylogenetic, chemotaxonomic, biochemical and electron microscopic data, demonstrated that strains FFA1^T and FFA3^T represented two novel species of the genus Ignatzschineria for which the names Ignatzschineria indica sp. nov. (type strain FFA1^T = DSM 22309^T = KCTC 22643^T = NCIM 5325^T) and Ignatzschineria ureiclastica sp. nov. (type strain FFA3^T = DSM 22310^T = KCTC 22644^T = NCIM 5326^T) are proposed.