Wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from Wohlfahrtia magnifica (Diptera: Sarcophagidae)
- 1 April 2008
- journal article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 58 (4), 976-981
- https://doi.org/10.1099/ijs.0.65324-0
Abstract
New Gammaproteobacteria were isolated from 3rd stage fly larvae of the parasitic fly Wohlfahrtia magnifica. Phylogenetic analysis of the new isolates showed that these bacteria belong to a distinct lineage close to Ignatzschineria larvae, which was originally isolated from the same species of fly. The low similarity values in 16S rRNA gene sequences (93.8–94.8 %), and differences in fatty acid profiles, RiboPrint patterns, MALDI-TOF mass spectra of cell extracts, and physiological and biochemical characteristics differentiate the isolates from the type strain of Ignatzschineria larvae (DSM 13226T), and indicate that our isolates represent a new genus within the Gammaproteobacteria. The major isoprenoid quinone of the strains is Q8, the major fatty acids are C18 : 1 and C14 : 0, and the predominant polar lipids are phosphatidylglycerol, phosphatidylethanolamine and phosphatidylserine. The G+C content of the DNA of the type strain is 44.3 mol%. The name Wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., is proposed for this novel genus and species. The type strain is S5T (=DSM 18708T=CCM 7401T).Keywords
This publication has 21 references indexed in Scilit:
- Demetria terragena gen. nov., sp. nov., a New Genus of Actinomycetes Isolated from Compost SoilInternational Journal of Systematic and Evolutionary Microbiology, 1997
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Agrococcus jenensis gen. nov., sp. nov., a New Genus of Actinomycetes with Diaminobutyric Acid in the Cell WallInternational Journal of Systematic and Evolutionary Microbiology, 1996
- Myiasis of Humans and Domestic AnimalsAdvances in Parasitology, 1995
- A standardized Gram staining procedureWorld Journal of Microbiology and Biotechnology, 1992
- Studies on the spectrophotometric determination of DNA hybridization from renaturation ratesSystematic and Applied Microbiology, 1983
- A rapid analysis for chemical grouping of aerobic actinomycetes.The Journal of General and Applied Microbiology, 1983
- A rapid method for the base ratio determination of bacterial DNAAnalytical Biochemistry, 1977
- The Quantitative Measurement of DNA Hybridization from Renaturation RatesEuropean Journal of Biochemistry, 1970
- The nature of discrepancies between hæmolysins in culture filtrates and plate hæmolysin patterns of staphylococciThe Journal of Pathology and Bacteriology, 1954