Electrophoretic Fabrication of Chitosan−Zirconium-Oxide Nanobiocomposite Platform for Nucleic Acid Detection
- 10 January 2011
- journal article
- research article
- Published by American Chemical Society (ACS) in Biomacromolecules
- Vol. 12 (3), 540-547
- https://doi.org/10.1021/bm1013074
Abstract
The present work describes electrophoretic fabrication of nanostructured chitosan−zirconium-oxide composite (CHIT−NanoZrO2) film (180 nm) onto indium−tin-oxide (ITO)-coated glass plate. This nanobiocomposite film has been explored as immobilization platform for probe DNA specific to M. Tuberculosis as model biomolecule to investigate its sensing characteristics. It is revealed that pH-responsive behavior of CHIT and its cationic skeleton is responsible for the movement of CHIT−NanoZrO2 colloids toward cathode during electrophoretic deposition. The FT-IR, SEM, TEM, and EDX techniques have been employed for the structural, morphological, and composition analysis of the fabricated electrodes. The morphological studies clearly reveal uniform inter-linking and dispersion of hexagonal nanograins of ZrO2 (30−50 nm) into the chitosan matrix, resulting in homogeneous nanobiocomposite formation. Electrochemical response measurements of DNA/CHIT−NanoZrO2/ITO bioelectrode, carried out using cyclic voltammetry and differential pulse voltammetry, reveal that this bioelectrode can specifically detect complementary target DNA up to 0.00078 μM with sensitivity of 6.38 × 10−6 AμM−1Keywords
This publication has 31 references indexed in Scilit:
- In Vivo Detection of Superoxide Anion in Bean Sprout Based on ZnO Nanodisks with Facilitated Activity for Direct Electron Transfer of Superoxide DismutaseAnalytical Chemistry, 2008
- Sol-gel derived nanostructured cerium oxide film for glucose sensorApplied Physics Letters, 2008
- Physical vapor deposited zinc oxide nanoparticles for direct electron transfer of superoxide dismutaseElectrochemistry Communications, 2008
- Immobilization of glucose oxidase on electrodeposited nickel oxide nanoparticles: Direct electron transfer and electrocatalytic activityBiosensors and Bioelectronics, 2007
- Nanowire-Based Electrochemical BiosensorsElectroanalysis, 2006
- Application of Nanoparticles in Electrochemical Sensors and BiosensorsElectroanalysis, 2006
- A Mediatorless Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Immobilized in Tin Oxide Sol‐Gel FilmAnalytical Letters, 2005
- Examination of Cholesterol oxidase attachment to magnetic nanoparticlesJournal of Nanobiotechnology, 2005
- Electrochemical biosensors utilising electron transfer in heme proteins immobilised on Fe3O4nanoparticlesThe Analyst, 2003
- Direct Electrochemistry and Nitric Oxide Interaction of Heme Proteins Adsorbed on Nanocrystalline Tin Oxide ElectrodesLangmuir, 2003